P-81: Effect of Ascorbic Acid and Albumin in The Sperm Selection Medium

Background: Several methods for sex selection of embryos have been used in research centers and infertility centers till now. Materials and Methods: In this research, the effect of medium, by placing the mouse (NMRI) spermatozoa in the medium containing certain concentrations of albumin and ascorbic acid, separation of spermatozoa containing X and Y chromosomes and doing in vitro fertilization (IVF) and embryo transfer was studied, In this case study, the mentioned mouse spermatozoa which was separated and located in IVF dish, near the same race mice oocytes by the injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (HCG) and were ovulation stimulated. Then, wards fetuses were transferred to the faster mother. Results: Statistical analysis was carried out by using the software package SPSS 16. The average percentage of male infants born in experimental groups is significantly different from the control groups (p<0.05). Out of fifty surrogate mother were pregnant by help of sperm separated in the presence of albumin and ascorbic acid. The achievement of questioned sex in the presence of 750 μm concentration of ascorbic acid was, 75.4% and in the control group was, 49.9%. Conclusion: The Addition of ascorbic acid and albumin caused decrease of peroxidation of lipid in the medium and it accepts the antioxidant of the vitamin and it makes radicals free from the sperm medium. This method is suitable for sperm containing X and Y chromosomes separation and male sex selection in fertile couple and non-manipulation of fetus is a safe method.